Purification, characterization of alkaline protease enzyme from native isolate Aspergillus niger and its compatibility with commercial detergents
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چکیده
منابع مشابه
Isolation, Production, Purification, Assay and Characterization of Alkaline Protease Enzyme from Aspergillus niger and its Compatibility with Commercial Detergents
Aspergillus niger is a highly potent fungus used in the production of alkaline protease. Extra cellular alkaline protease was purified from A. niger in a twostep procedure involving ammonium sulphate precipitation and Sephadex G100 column chromatography. The molecular mass of the enzyme was determined to be 60 kDa by SDS-PAGE. The enzyme activity was also analyzed by zymogram with gelatin. The ...
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An alkalophilic fungal strain, Aspergillus versicolor PF/F/107 isolated from poultry farm produced alkaline protease at 40C and 9.0 pH. Protease showed compatibility with commercial detergents and retained 50-76% of its original activity at 40C in the presence of detergents in the following order: Ghadi, Ujalla, Surf excel, Ariel and Tide indicating its suitability for application in detergen...
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The goal of this research was to isolate and identify the thermostable alkaline protease producing bacteria among several native Iranian microorganisms. At the end of screening program, a Bacillus subtilis BP-36 strain producing thermophilic alkaline protease was isolated from a hot spring in Ardebil province. The target enzyme was purified using a one-step Aqueous two-phase systems (ATPS) prot...
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This work reports the production of a novel alkaline protease from the fungus Aspergillus niger. The protease was purified from the culture supernatant to homogeneity using ammonium sulfate precipitation, Sephadex G-150 gel filtration and DEAE-sepharose ion exchange chromatography with a 13.9-fold increase in specific activity. The molecular weight of the enzyme was estimated to be 32 kDa on SD...
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Endoglucanase B (EGLB) derived from Aspergillus niger BCRC31494 has been used in the food fermentation industry because of its thermal and alkaline tolerance. It was cloned and expressed in Pichia pastoris. According to sequence analysis, the gene open reading frame comprises 1,217 bp with five introns (GenBank GQ292753). According to sequence and protein domain analyses, EGLB was assigned to g...
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ژورنال
عنوان ژورنال: Indian Journal of Science and Technology
سال: 2008
ISSN: 0974-6846,0974-5645
DOI: 10.17485/ijst/2008/v1i7.8